Composite
Part:BBa_K1030001:Design
Designed by: Wei Huang Group: iGEM13_SUSTC-Shenzhen-A (2013-09-16)
J23101->tetA-asv+sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1106 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 960
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 406
Illegal NgoMIV site found at 566
Illegal NgoMIV site found at 934 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1273
Design Notes
We use a series of synthetic constitutive promoter to tune the expression of tetA, which in turn tune the extracellular Nickel tolerance level. sfGFP is used to monitor tetA expression.
Source
J23101 and sfGFP are from iGEM, tetA is from pBR322.